The women's 4 × 400 metres relay event at the 2016 African Championships in Athletics was held on 26 June in Kings Park Stadium.
Results
| Rank | Nation | Competitors | Time | Notes |
|---|---|---|---|---|
| South Africa | Jeanelle Griesel, Wenda Nel, Justine Palframan, Caster Semenya | 3:28.49 | CR, NR | |
| Nigeria | Omolara Omotosho, Regina George, Yinka Ajayi, Patience Okon George | 3:29.94 | ||
| Kenya | Jacinta Shikanda, Maureen Jelagat, Maureen Thomas, Margaret Wambui | 3:30.21 | ||
| 4 | Botswana | Christine Botlogetswe, Lydia Jele, Galefele Moroko, Goitseone Seleka | 3:31.54 | |
| 5 | Ghana | Agnes Abrocqua, Shawkia Iddrisu, Bless Dupeh, Akua Obeng-Akrofi | 3:40.09 | |
| Cameroon | DNS |